Sequencing single B cells

Carrier Determination in a Hemophilia B Family Using Single Strand Conformation Polymorphism (SSCP) and Sequencing

Direct high‐throughput amplification and sequencing of immunoglobulin genes from single human B cells

Single cell Ig gene amplification and sequencing has been widely used for the molecular and functional assessment of human antibody repertoires and has led to the identification of recombinant monoclonal antibodies with therapeutic potential against diverse pathogens [1–3]. Due to the high reagent and Sanger sequencing costs, these antibody-cloning strategies are limited to the analysis of rela...

I-37: Establishing High Resolution Genomic Profiles of Single Cells Using Microarray and Next-Generation Sequencing Technologies

The nature and pace of genome mutation is largely unknown. Standard methods to investigate DNA-mutation rely on arraying or sequencing DNA from a population of cells, hence the genetic composition of individual cells is lost and de novo mutation in cell(s) is concealed within the bulk signal. We developed methods based on (SNP-) arraying and next-generation sequencing of single-cell whole-genom...

Sequencing Single Cells Reveals Sequential Stem Cell States.

Understanding the molecular mechanisms underlying activation of quiescent neural stem cells (NSCs) is complicated by heterogeneity in coexisting NSC pools. Two papers in this issue of Cell Stem Cell (Llorens-Bobadilla et al., 2015; Shin et al., 2015) report sequencing of single NSCs, providing insights into the transition from quiescence to activation and highlighting common themes in NSCs from...

Genome sequencing and phenotypic analysis of single cells in cancer

Relatively little is known about metastatic cancer. Ninety-nine percent of cancer genome profiling is done on primary tumors; yet, metastatic cancer is responsible for ninety percent of cancer-related deaths. The underlying challenge is that metastatic cancer is difficult to sample: surgical resections are less common, metastatic biopsies are not part of clinical routine, and such invasive biop...